Application notes, comparison guides & how-to material.
Technical material for downstream-processing teams working on AAV, mRNA, monoclonal antibodies and sensitive biologic formats.
Technology
What is rapid cycling chromatography?
Running a small column many times instead of a large one once — how bioprocess intensification works in a capture step, what it costs in cycles and cleaning, and when a packed bed is simply the better answer.
Read →Feed preparation: how far do you have to clarify?
Centrifugation, depth filtration, membrane filtration — what each one removes, how far down the train you actually have to go, and what every stage costs you in yield. The question that comes up in almost every first conversation.
Read →Hydrophobic interaction chromatography: how it works and when to use it
HIC binds at high salt and elutes as the salt falls — the reverse of ion exchange, and mild enough that the protein stays folded. What the salt actually does, how to pick a ligand, and where the step earns its place.
Read →How do you read a dynamic binding capacity figure?
A DBC number without its conditions is not a number. The seven conditions that have to match before two figures can be compared, why bed height matters as much as residence time, the arithmetic mistake that overstates capacity by ten per cent — and when it is worth measuring on your own feed.
Read →- Article
Monolithic chromatography: what a monolith column is and when it helps
Channels instead of pores, convection instead of diffusion: how a chromatography monolith works, which chemistries it carries, and where a packed bed is still the better answer. MonoCore™ runs 15 µm channels at about 8 column volumes per minute.
Read → - Comparison Guide
Alternatives to membrane adsorbers — what else runs at convective speed?
If a membrane adsorber is not the right fit, the closest alternative in the same speed class is a monolith. Both are convective media. MonoCore™ is a modified cellulose monolith available as Q and S, HIC with butyl and phenyl ligands, Protein A and Protein G, with a 15 µm channel size in 1.8 mL and 5.4 mL capsules with UNF 10-32 connectors.
Read → - Article
Why chromatography media foul when you load a biological feed
Where fouling actually happens: the mechanisms, how to tell them apart from the pressure curve, and why channel size decides robustness. Adsorptive membranes run at roughly 0.8 to 3 µm, a MonoCore™ capsule at 15 µm — three to twenty times wider, in one continuous flow path.
Read → - Article
What monoliths are made of — and what the backbone actually decides
Three material families dominate preparative monolithic chromatography: polymethacrylate, styrenic or methacrylic polyHIPE foams, and cellulose. The backbone decides two different things, and they are worth keeping apart. It sets the surface your molecule touches — hydrophilic from the start, or hydrophilic only after modification. And through the manufacturing route it sets th
Read → - Article
Convective vs diffusive mass transport in chromatography
Why packed-bed resins hit a ceiling on flow rate, how convective formats move it, and what that changes for cycle time, peak shape and method transfer.
Read → - Comparison Guide
Monolith vs membrane chromatography: a practical comparison
Both monoliths and membrane adsorbers move beyond diffusion-limited packed beds, but they solve the throughput-vs-resolution trade-off in different ways. Here's how they compare on hydraulics, resolution, scale-up and operating cost.
Read → Lot-to-lot consistency in monolith columns: what to ask for
A monolith is cast, not packed — which changes what batch-to-batch variation means and how it is measured. What a supplier can show you, which tests actually detect a difference, and what belongs on a certificate of analysis.
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AAV / Viral Vectors
AAV purification: the protocol, step by step
From harvest to formulated vector: lysis or supernatant, clarification, affinity capture, the full/empty separation on anion exchange, polishing and concentration — with the decision behind each step and where the route differs by serotype.
Read →- How-to Guide
Which chromatography media are suitable for AAV polishing?
After affinity capture, AAV polishing removes residual host cell protein and DNA and enriches full capsids over empty ones. Because an AAV capsid is roughly 25 nm across, it barely enters the pores of a packed-bed resin — so convective media are the usual choice. MonoCore™ Q and MonoCore™ S are modified cellulose monoliths with a 15 µm channel size in 1.8 mL and 5.4 mL capsules with UNF 10-32 connectors.
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mAb & Bispecifics
Antibody purification: how the standard route works
Capture, virus inactivation, polishing, virus filtration, formulation — the route that gets a monoclonal antibody from harvest to drug substance, the decision behind each step, and where it differs for hybridoma, bispecific and Fc-fusion formats.
Read →How do you elute from Protein A without acid?
Nine routes away from low-pH elution — and only three of them remove the acid entirely. Mild-elution ligands at pH 4.3–5.0, amino-acid and arginine buffers, glycols, salt and calcium systems, detergents, alternative ligands, mixed-mode capture, and light-controlled release at neutral pH (Solaris® Protein A, Beta). What each one costs, and where each one stops.
Read →- How-to Guide
HIC monoliths: how to remove aggregates on a monolith column
How hydrophobic interaction works in a monolith, when bind-and-elute beats flow-through, how Butyl and Phenyl differ — on MonoCore™ HIC with 15 µm channels in 1.8 and 5.4 mL — and where the format does not help.
Read → - How-to Guide
How do you purify an antibody from hybridoma supernatant?
Which ligand binds your subclass — Protein G for mouse IgG1, Protein A for dog and cat — how to handle bovine IgG from the serum, and the capture cycle with buffers, volumes and step times on MonoCore™ capsules.
Read → - How-to Guide
Protein A chromatography: how to run a capture cycle
The Protein A capture cycle with buffers and volumes: feed preparation, equilibration, load, wash, elution and neutralisation, the virus inactivation hold, leached ligand and cleaning — and what the same ten steps look like on a MonoCore™ Protein A capsule.
Read → - How-to Guide
Protein A column troubleshooting: symptoms and what to check
Low yield, aggregate in the eluate, host-cell protein carryover, rising back pressure, capacity loss: the likely cause for each symptom, the check that confirms it, the fix — and the two checks that differ on a MonoCore™ capsule.
Read → - How-to Guide
Protein A elution buffer: glycine, citrate or acetate?
Which buffer to elute a Protein A column with, at which pH, and what it costs in aggregate: glycine, citrate, acetate and arginine compared — plus why a shorter residence time, seconds on a MonoCore™ capsule, shortens the acid exposure itself.
Read → - How-to Guide
How do you regenerate and clean a Protein A column?
Regeneration, cleaning, sanitisation and storage are four procedures with four purposes. What each does, what limits the service life of a Protein A column, how to measure the decline — and what is specified for a MonoCore™ affinity capsule.
Read → - How-to Guide
Protein G capture for murine IgG1 — which media are available at lab and pilot scale?
Which ligand and which format for mouse monoclonals: why murine IgG1 needs Protein G, what exists at lab and pilot scale, and where MonoCore™ Protein G at 15 µm fits between resin and membrane.
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