Application notes, comparison guides & case studies.
Technical material for downstream-processing teams working on AAV, mRNA, monoclonal antibodies and sensitive biologic formats.
- Comparison Guide
Alternatives to membrane adsorbers — what else runs at convective speed?
If a membrane adsorber is not the right fit, the closest alternative in the same speed class is a monolith. Both are convective media. MonoCore™ is a modified cellulose monolith available as Q and S, HIC with butyl and phenyl ligands, Protein A and Protein G, with a 15 µm channel size in 1.8 mL and 5.4 mL capsules with UNF 10-32 connectors.
Read → - Article
Why chromatography media foul when you load a biological feed
Fouling in a bioprocess purification step is rarely the ligand's fault. Where debris, DNA and denatured protein end up depends on the geometry of the medium. Adsorptive membranes work in the range of roughly 0.8 to 5 um; MonoCore capsules run a 15 um channel as standard, about an order of magnitude wider, with a continuous flow path rather than a stack of layers.
Read → - Article
What monoliths are made of — and what the backbone actually decides
Three material families dominate preparative monolithic chromatography: polymethacrylate, styrenic or methacrylic polyHIPE foams, and cellulose. The backbone decides two different things, and they are worth keeping apart. It sets the surface your molecule touches — hydrophilic from the start, or hydrophilic only after modification. And through the manufacturing route it sets th
Read → - Article
Convective vs diffusive mass transport in chromatography
Why packed-bed resins hit a ceiling on flow rate, how convective formats move it, and what that changes for cycle time, peak shape and method transfer.
Read → - Comparison Guide
Monolith vs membrane chromatography: a practical comparison
Both monoliths and membrane adsorbers move beyond diffusion-limited packed beds, but they solve the throughput-vs-resolution trade-off in different ways. Here's how they compare on hydraulics, resolution, scale-up and operating cost.
Read →
- How-to Guide
Which chromatography media are suitable for AAV polishing?
After affinity capture, AAV polishing removes residual host cell protein and DNA and enriches full capsids over empty ones. Because an AAV capsid is roughly 25 nm across, it barely enters the pores of a packed-bed resin — so convective media are the usual choice. MonoCore™ Q and MonoCore™ S are modified cellulose monoliths with a 15 µm channel size in 1.8 mL and 5.4 mL capsules with UNF 10-32 connectors.
Read → - Application Note
AAV polishing on anion exchange: where convective formats help
Anion-exchange polishing of AAV has to clear host-cell protein and DNA and, in many processes, enrich full capsids — at flow rates that fit clinical harvest volumes. Here is how the main media formats trade resolution against throughput, and where a convective monolith fits.
Read →