MonoCore™ S
Cation exchange monolith column
Supplied for research and process development.
Strong and weak cation exchange in monolith format — for mAb and bispecific polishing. Resin-class peak resolution at membrane-class throughput.
Specifications
MonoCore™ S is a modified cellulose monolith with a 15 µm (standard), custom 10–150 µm on request standard channel size, supplied as pre-assembled 1.8 mL and 5.4 mL capsules with UNF 10/32 ports. Larger formats are available on request, and the capsule connects directly to a standard FPLC system.
- Chemistry
- Strong & Weak Cation Exchange
- Matrix
- Modified cellulose
- Format
- 1.8 mL · 5.4 mL · larger on request
- Channel size
- 15 µm (standard), custom 10–150 µm on request
- Connectors
- Standard FPLC (UNF 10/32)
Detailed performance data are available on request.
Frequently asked questions
Do I need to filter the feed before loading?
- Yes — the feed has to be clarified, as for any chromatography step. Centrifugation followed by depth filtration is the usual route, and it is what protects a column from the debris that drives back-pressure up over a run. Whether you add a 0.22 or 0.45 µm step immediately before the column is a separate decision: it matters most where the narrowest part of the flow path is the gap between packed particles. A 15 µm open channel leaves more margin there, but a feed that has been standing and has begun to precipitate will still cause trouble. Match the filter train to the feed you actually have rather than to the one from the last project — every filtration stage also costs you product through adsorption.
Related products
- AEX
MonoCore™ Q
Strong and weak anion exchange in monolith format — for capture and impurity removal in AAV polishing, pDNA capture, and host-cell-protein clearance. Resolution that lets you use it for capture, not only polishing.
- HIC
MonoCore™ HIC
Hydrophobic interaction chromatography (HIC) in monolith format — available with Butyl or Phenyl ligands. Frequently used for aggregate removal, with tight aggregate–monomer separation at process flow rates.
- Protein A
MonoCore™ Protein A
Protein A monolith column for antibody capture: recombinant Protein A on a modified cellulose monolith, for IgG and Fc-bearing formats. Cleaning conditions are available on request.