Custom Affinity
Custom affinity monolith column
Supplied for research and process development.
Tailored affinity ligands coupled to the MonoCore™ matrix on request — talk to a scientist.
Specifications
Custom Affinity is a modified cellulose monolith with a 15 µm (standard), custom 10–150 µm on request standard channel size, supplied as pre-assembled 1.8 mL and 5.4 mL capsules with UNF 10/32 ports. Larger formats are available on request, and the capsule connects directly to a standard FPLC system.
- Chemistry
- Affinity
- Matrix
- Modified cellulose
- Format
- 1.8 mL · 5.4 mL · larger on request
- Channel size
- 15 µm (standard), custom 10–150 µm on request
- Connectors
- Standard FPLC (UNF 10/32)
Detailed performance data are available on request.
Frequently asked questions
Do I need to filter the feed before loading?
- Yes — the feed has to be clarified, as for any chromatography step. Centrifugation followed by depth filtration is the usual route, and it is what protects a column from the debris that drives back-pressure up over a run. Whether you add a 0.22 or 0.45 µm step immediately before the column is a separate decision: it matters most where the narrowest part of the flow path is the gap between packed particles. A 15 µm open channel leaves more margin there, but a feed that has been standing and has begun to precipitate will still cause trouble. Match the filter train to the feed you actually have rather than to the one from the last project — every filtration stage also costs you product through adsorption.
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