MonoCore™ Protein A
Protein A monolith column for IgG capture
Supplied for research and process development.
Protein A monolith column for antibody capture: recombinant Protein A on a modified cellulose monolith, for IgG and Fc-bearing formats. Cleaning conditions are available on request.
Specifications
MonoCore™ Protein A is a modified cellulose monolith with a 15 µm (standard), custom 10–150 µm on request standard channel size, supplied as pre-assembled 1.8 mL and 5.4 mL capsules with UNF 10/32 ports. Larger formats are available on request, and the capsule connects directly to a standard FPLC system.
- Chemistry
- Protein A
- Matrix
- Modified cellulose
- Format
- 1.8 mL · 5.4 mL · larger on request
- Channel size
- 15 µm (standard), custom 10–150 µm on request
- Connectors
- Standard FPLC (UNF 10/32)
Detailed performance data are available on request.
Frequently asked questions
Do I need to filter the feed before loading?
- Yes — the feed has to be clarified, as for any chromatography step. Centrifugation followed by depth filtration is the usual route, and it is what protects a column from the debris that drives back-pressure up over a run. Whether you add a 0.22 or 0.45 µm step immediately before the column is a separate decision: it matters most where the narrowest part of the flow path is the gap between packed particles. A 15 µm open channel leaves more margin there, but a feed that has been standing and has begun to precipitate will still cause trouble. Match the filter train to the feed you actually have rather than to the one from the last project — every filtration stage also costs you product through adsorption.
Which antibody formats does Protein A capture?
- Human IgG1, IgG2 and IgG4 bind well, as do Fc-bearing formats such as bispecifics, Fc-fusions and ADCs. Human IgG3 and mouse IgG1 bind weakly — those are the cases for Protein G.
What elution conditions are used?
- The classical low-pH step: Typically 0.1 M glycine-HCl or 0.1 M acetic acid around pH 3.0, collected into 1 M Tris-HCl pH 8.5 — these are the conditions in common use for Protein A rather than a specification of this product; the conditions we characterise under are in the datasheet so the product is neutralised as it elutes. Keep the hold time at low pH short.
How is the capsule cleaned?
- The cleaning conditions for MonoCore™ Protein A are available on request. Those figures describe the housing and the matrix. On an affinity capsule they are not the operating limit: the ligand is the sensitive part, and it is what decides the cleaning conditions. Protein ligands lose capacity in strong alkali, so do not infer a caustic CIP regime from the housing figures — the conditions we characterise under come with the datasheet.
What binding capacity should I expect?
- The datasheet states the capacity together with the conditions it applies to. Capacity is feed-specific: measure it on your own molecule at the residence time you intend to run.
Does it fit my system?
- UNF 10/32 coned ports for 1/16″ tubing, 1.8 mL and 5.4 mL formats, recommended flow 15 and 45 mL/min respectively, maximum 0.8 MPa before the capsule. No re-plumbing of a standard FPLC system.
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