MonoCore™ HIC

Frequently asked questions

Do I need to filter the feed before loading?

Yes — the feed has to be clarified, as for any chromatography step. Centrifugation followed by depth filtration is the usual route, and it is what protects a column from the debris that drives back-pressure up over a run. Whether you add a 0.22 or 0.45 µm step immediately before the column is a separate decision: it matters most where the narrowest part of the flow path is the gap between packed particles. A 15 µm open channel leaves more margin there, but a feed that has been standing and has begun to precipitate will still cause trouble. Match the filter train to the feed you actually have rather than to the one from the last project — every filtration stage also costs you product through adsorption.

Which ligands are available?

Butyl and Phenyl, both on the same modified cellulose matrix and in the same capsule format, so the two can be screened on identical hardware.

Bind-and-elute or flow-through for aggregate removal?

Both are in use. Flow-through needs less buffer and is simpler at scale; bind-and-elute with a descending salt gradient gives more control when aggregate levels are high or the separation is tight.

What is the channel size?

15 µm as standard; alternative sizes on request. The size is fixed within one monolith and chosen per column.

Does it fit my system?

The capsule has UNF 10/32 coned ports for 1/16″ tubing and runs on a standard FPLC system without re-plumbing. Formats are 1.8 mL and 5.4 mL, larger on request.

What binding capacity should I expect?

Capacity depends on the molecule, the salt condition and the residence time you run, so it is measured on the feed rather than taken from a table. The datasheet states the conditions we measure under; our guide to reading a dynamic binding capacity figure explains what has to match before that number transfers to yours.