Affinity chromatography— controlled by light, not chemistry.
PureLight® replaces the elution step of classical affinity chromatography with a light pulse. Bind under red light, elute under blue light at neutral pH. No acid hold, no aggregation triggers — even on sensitive formats.
How PureLight® works
The affinity ligand carries an azobiaryl photoswitch built into a stable 3-helix-bundle scaffold. Visible-light isomerisation between cis and trans toggles the binding affinity reversibly — without harming the target. The matrix is the same hydrophilic cellulose monolith that powers MonoCore™. The platform is designed to extend beyond Protein A to other affinity ligands.
Red — bind
(630 nm)Photoswitch sits in its high-affinity conformation. Target binds at native pH and ionic strength.
Blue — elute
(480 nm)A blue-light pulse triggers reversible cis/trans isomerisation. Affinity collapses; product elutes in your buffer of choice.
Reversible
Demonstrated over 100 IgG binding and elution cycles.
Neutral elution
No low-pH hold, no high-salt elution buffer — preserves sensitive formats (bispecifics, ADCs, viral vectors).
PureLight® Beta — open for partner programs.
We are open to working with biotech and CDMO partners on application-specific evaluations of PureLight® — particularly for bispecifics, ADCs, viral vectors, and other sensitive formats where classical elution is limiting. If that fits your pipeline, get in touch.